Lymphocyte typing focuses on identifying the numbers and relative percentages of lymphocytes in an individual's bloodstream. Lymphocytes, primarily T cells and B cells, are types of white blood cells, the underlying supports of the immune system in the bloodstream.
Determining the numbers and relative percentages of T cells and B cells provides information on the state of a person's immune system. By comparing these values to normal numbers and percentages, the presence of disease and the side effects of certain drugs can be revealed. Lymphocyte typing can also show whether a person has been exposed to certain poisonous substances.
To do a white blood cell count, a small amount of blood is drawn from a vein. The total number of white blood cells is calculated, either through microscopic examination of a blood smear or by using automated counting equipment. For a white blood cell count with differential, 100 white blood cells are counted and the proportion of each type is calculated. Since T cells and B cells have similar appearances, a differential can only give the proportion of lymphocytes in the blood, not the proportion of specific lymphocyte types.
For more specific information on B cells and T cells, it is necessary to divide the blood into its separate components. In this procedure, a tube of blood is placed in a centrifuge, a piece of equipment that spins the tube in circles at high speed. The force generated by the spinning causes the various elements in the bloodstream to settle at different levels of the tube.
The lymphocytes are extracted from the tube and treated with special dyes, or stains. Each stain is equipped with an antibody portion that adheres to a specific type of lymphocyte, such as a B cell or a T cell. The stains make the cells visible to an automated counting machine, called a flow cytometer. Based on the number of times the machine detects a particular stain, it can calculate the number of the associated cell type. This procedure can also be used to classify T cells and B cells into their subtypes.
Julia Barrett, The Gale Group Inc., Gale, Detroit,